Technique of microinjection in axons of nerve cells, surrounded by dense glial sheath, using ultrasound vibration of microcapillary

Abstract

Technique of microinjection in axons of nerve cells, surrounded by dense glial sheath, using ultrasound vibration of microcapillary

M.S. Kolosov, E.V. Duz., V.K. Yacenko

Incoming article date: 18.12.2013

Intracellular microinjection - a method of direct introduction of cellular organelles and solutions of substances into living cells. The technique of microinjection into nerve cells by puncturing their processes - axons , which are often surrounded by a dense glial sheath, are described. To puncture the sheath a special injector transmitting microvibrations of ultrasonic frequency on the injection capillary was designed. The results of using the proposed microinjection technique for introducing fluorescent marker in large axons of model object - crayfish ventral nerve cord, to visualize the morphology of axons, were shown.

Keywords: intracellular microinjection, neuron, axon, glial sheath, ultrasound vibration, piezoelectric transducer